Application

Western blot: 1-10 µg/mL using ECL.

ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide per well (Catalog number AG217).

Optimal working dilutions must be determined by the end user.

Research CategoryInflammation & Immunology

Anti-Histamine Receptor 4 Antibody, C-terminus detects level of Histamine Receptor 4 & has been published & validated for use in ELISA & WB.

Research Sub CategoryInflammation & Autoimmune Mechanisms

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A 17 amino acid peptide sequence within the cytoplasmic, C-terminus of human HR4 (Oda et al. 2000; Nakamura et al. 2000).

Epitope: C-terminus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA.

Specificity

Recognizes human Histamine Receptor 4 (HR4). The immunogen shows no significant sequence homology with other histamine receptors.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.